Texas Beach Scene--somehow I earned a PhD in three years |
In early January 1974, I flew down to
Texas from my family home in New Jersey. My one suitcase was filled with woolen
winter clothes, leather boots, and only a few summer things. I brought my
pillow and a typewriter. Professor Pat Parker
picked me up at the airport in his old VW bug, rusted out on the sides. He was
a modest looking man with a small mustache, a bit of a limp, and shaggy brown
hair. Even as the Director of the Institute, he wore old khakis and an
un-ironed short sleeve shirt with no tie. I had formed a different picture of
how he looked based on his Science papers that I had read at Penn State.
We drove to Port Aransas on Mustang
Island, where the Marine Science Institute was located, with the final portion
of the journey via ferry. Coming from urbanized Southern New Jersey, Port
Aransas seemed like a town literally at the end of the earth for me. The next
day I met Professor Chase Van Baalen. Chase was often mistaken for one of the
maintenance staff. A short fellow with a crew cut, Van Baalen smoked a pipe,
and was known for shoving his lit pipe deep into the pockets of his jeans. His
right hand lab assistant, Rita O’Donnell, took care of the lab glassware and
culturing facility for Chase. She was a crusty Port Aransan, who also owned and
managed a popular motel with her husband. Parker, Van Baalen, and Rita formed
the core of my scientific mentors at the Institute.
My first house in Port Aransas was a
two-room bungalow within walking distance of the Marine Lab. I bought an old
bicycle that I used to get to town for shopping. It was the first time I had
lived alone. I needed to buy plates, pots and pans, silverware in one of the
two grocery stores on the Island. Without a car, I couldn’t get to the larger
supermarkets and stores on the mainland. Meals were generally one-pots. My
specialty was rice-a-roni with vegetables and an egg scrambled in at the end.
Life was pretty simple.
My first bungalow in Texas, this photo taken in 2011 |
UT's Marine Science Institute |
In 1974 when I started grad school,
the Vietnam War was winding down. Mustang Island, Port Aransas, Texas, where
the Marine Science Institute was located saw an influx of returning soldiers
who had discharged out of the service and gravitated to South Texas for a
number of reasons, one of which was easy access to a pipeline of heroin coming
in from Mexico. Jobs were easy to find on shrimp boats, in construction, or on
oilrigs in the Gulf. Being an academic in this small town meant you were in the
minority and a world away from the city life of Austin, the main Univ. of Texas
campus.
I met many of these vets in the
local “Port A” bars, the only social places on the island where people hung out
after work. Compared to State College, Pennsylvania or New Jersey the folks
that lived on Mustang Island were a rougher crowd than I was used to. Shorty’s
bar down on the Flats of Port Aransas near the docks was a rough and tumble
place run by a 70 year old woman, Shorty, who kept a pool stick and a pistol
behind the bar to keep order. A gruff bartender, she knew all of her customers
and lent them a $10 bill when they needed it or gave a free beer to someone who
couldn’t afford the $1 that she charged for a draft. Sadly, a drunk customer
who didn’t understand her brand of “humor” murdered Shorty in her bar in the
1990s.
Next door to Shorty’s was The Party
House, owned and operated by a former Marine Science grad student and some
buddies. It was a raucous place filled to the brim with the younger hippy
crowd. They served pizzas—the only place in town where you could get one. It
was common for the rowdy customers to snag pieces off of your pizza as
the waiter brought it to your table. By midnight, everyone howled with the
music, yelled “Yeeha!” and danced wildly. I learned how to dance the Texas
classic dance--the Cotton Eyed Joe--there, which generally erupted in the wee
hours.
Port Aransas in those days boasted
one of the widest, cleanest beaches on the Gulf Coast. The sand was firm enough
to drive on, so the beach was a scene of Texans cruising the surf line in their
fancy pickup trucks and Cadillacs—a far cry from the Jersey Shore where I had
grown up. People brought their BBQ grills with them along with coolers of Lone
Star, Pearl, and Shiner beer. By nightfall, fireworks lit up the sky and the
smell of marijuana wafted from most of the vehicles. Camping was permitted on
the beach either in RVs, tents, or makeshift encampments around trucks.
Although sheriffs cruised the beach day and night, it was decidedly a “scene”,
which contrasted to the quiet, rarified atmosphere at the Institute.
Soon after I arrived I met Carlos
Jack Estep in the Sail Club saloon, a hangout for the country-western crowd.
Jack used the old line, “What’s a nice girl like you doing in a place like
this?” Country music blared on the jukebox and we danced to “Behind Closed
Doors,” a popular ballad by Charlie Rich. He was thirteen years older than me,
had been married before, and was known all over Port Aransas as Jack the Sign
Painter. With a shock of straight blonde hair and a sly smile, he had many of
the local Port Aransas lasses following him around. He’d served in the US Air
Force, had had a life as a businessman in San Antonio, but now was more of a
hippie who traveled to Central America and took it easy. At that time, I had no
aspirations of becoming a middle class person like my parents. The idea of an
alternative life style was attractive.
Alone and not yet in step with my
lab colleagues, I was swept into a whirlwind romance. One month later, we
eloped and got married on a party boat that took tourists out to fish in the
Gulf for the day. At the time, I thought this was very romantic and
adventurous. I was out on my own and making decisions. Years later, I could see
that a wedding without family is a red flag for future trouble. At that time,
young people were choosing to live in different ways than their parents,
typically without marriage. I thought my new moniker as a “wife” was cool, but
also risky and a bit reckless. I’d never done anything reckless in my life. I
took a chance here. It was a very heady time.
I kept the hasty marriage a secret
from my family and friends until at least a month later, guessing that the
others might not be as thrilled about it as I was. I ended up writing to my
parents telling them about the marriage. My family was ripped apart by the
news. My mother and my sister Barb flew down to Texas for Barb’s spring break
and met Jack. He was on his best charming behavior, showing them some of the
local Texas sites, barbequing great meals, and telling jokes. The shock of a
secret wedding was smoothed over.
As a newly married woman, I could
concentrate fully on my work in the lab rather than pay any attention to the
interlab gossip about who was dating whom. I arrived at work by 8 am, kept my
head down until 5:30 pm, and then pedaled my bicycle back to the little house
we lived in for the first half-year. Grad students were required to move to
Austin for two semesters to take formal classes, so we were uprooted from the
beach life to big city Austin in August 1974. Rather than rent an apartment, we
had the “brilliant” idea to take an old cab-over camper and convert it into a
houseboat that would reside at a marina on Lake Travis, in the hill country
west of Austin. Jack started the remodel, but with just about everything he
started, his work rarely lived up to expectations and frequently never was
completed. Our “house boat” became a small land trailer that lacked a bathroom.
The only running water was a hose connecting to a small sink in a tiny kitchen.
We lived in this modified camper for not quite three years, occupying various
trailer parks in Port Aransas after returning from Austin.
On main campus in Austin, I spent
fall semester as a teaching assistant for Oceanography, earning about
$300/month. Jack was a sign painter by profession, with no connections in
Austin, so my salary provided our total support. We qualified for food stamps,
which kept us going. I also had a side business making jewelry out of used, old
eye glass lenses, something I had been doing since I was a college student.
Between my studies, I made pendants and pins adorned with laminated famous
paintings that Jack sold on the streets at craft markets. A necklace sold for $4
and a pin for $3. We made just enough to get by. In winter 1975, I needed to
spend time studying for my qualifying exam, a nerve-wracking oral examination
designed to find out everything that you didn’t know. I also completed my
course work and passed the German foreign language exam by reading a hefty tome
by Otto Warburg, a German physiologist. No question we were living in poverty,
but that mattered little because I was consumed by my work.
Returning to Port Aransas at the end
of May, there was nowhere to park the “houseboat” so we lived on the beach for
a couple of weeks before finding out about a small tourist motel with eight
units that needed to hire managers. We applied and became managers of the Gulf
Beach Resort Hotel situated half a block from Port Aransas’ white sand beaches.
We were then able to move into a small apartment with air conditioning for the
summer.
The Gulf Beach resort motel (in 2012--now pink) |
Before we’d moved back to Port
Aransas, we purchased a 1966 International Scout that had been used as an ice
cream truck in Austin. Down in Port A, Jack painted the truck with pink and
white stripes with the Pink Panther cartoon character eating a popsicle on each
side. There was already one ice cream
truck selling on the beach so we named our truck “The Other Ice Cream Truck.”
We purchased a second large floor freezer and once a week the ice cream
supplier from Corpus Christi loaded it up with various ice cream products. We
hired two young folks to drive the truck---a buxom blonde who wore a bikini and
a good-looking blonde surfer dude with a charming easy personality. It was not
long before I became the number one ice cream dealer in South Texas!
I started my PhD project in summer of
1975 with funding from a NASA Exobiology grant working with Parker, Van Baalen,
and microbiologist/enzymologist F. Robert Tabita. My PhD research was to purify
the enzyme key in fixing carbon dioxide in photosynthesis—Rubisco--from various
organisms, then carry out a series of complicated experiments to measure how
carbon isotope patterns were modified by Rubisco.
Harmon Craig—one of the earliest
pioneers (an Isopope to be sure) in stable isotope research--wrote the first
blockbuster paper on “stable carbon isotopes in everything” from diamonds to an
unwitting discovery of a different type of grass with a unique carbon isotope
signature (Craig, 1953). His later work tracked major carbon isotope
systematics in the world’s oceans. At the same time, Melvin Calvin and A. A.
Benson were using radiocarbon to track the pathways of carbon fixation (Calvin
and Benson, 1948) that would eventually garner Calvin a Nobel Prize. A paper by
Park and Epstein (1960) linked the two types of studies and measured, for the
first time, the carbon isotope fractionation by the CO2 fixing
enzyme Rubisco. Using a purified enzyme, bicarbonate, and ribulose
1,5-bisphosphate (RuBP), they calculated an isotope fractionation indicating
the lighter isotope of carbon was favored over the heavier one during the
reaction. Not much was known about the structure and function of Rubisco at this
time.
Work in the late 1960s and early 1970s
on the structure and function of Rubisco brought new interest to the carbon
isotope fractionation studies in plants. Bob Tabita, then a recent PhD and
postdoc, brought his skills in purifying Rubisco as an Assistant Professor at
the University of Texas. His work centered on isolating and describing the
different forms of bacterial Rubisco enzymes—their structures, kinetics, and
activations. Rubisco from higher plants has a very large molecular weight (550,00
daltons) and is composed of 8 large subunits and 8 small subunits.
In June at the Laboratory, I took my
oral exam and was asked questions about lipid (fat) metabolism, a topic I did
not study thoroughly. After writing an essay on that topic, the exam was passed
and I became a PhD candidate. I had to tell my professor Chase Van Baalen that
I had another job, or two. His response was, “As long as this doesn’t affect
your work and you’re here Monday through Friday, it’s fine with me.” I kept my
nose to the grindstone. Van Baalen did not believe in chairs in the laboratory,
so I stood all day at my assigned lab bench. He also did not believe in
automated biochemistry equipment, so all of my enzyme purifications were
carried out by manually advancing a rack of test tubes every 5 minutes.
Van Baalen had several folksy
expressions he liked to use. If you were found sitting at your desk, instead of
at your lab bench, he’d pop his head in to your office and ask, “Wanta learn a
trade?” He wore a slightly devilish little smile. The answer you needed to give
was, “Sure.” One time when I was asked this, I was taught how to change the oil
in a vacuum pump. Changing a pump’s oil is a messy, slimy job that nearly
everyone avoids until it absolutely has to be done. I learned two things from
him—a technical skill I have used for my entire career—and a management tool to
get timid students to try something new. After a couple of months of figuring
things out how things worked in the Van Baalen laboratory, by the fall semester
I was well into my research.
After a
couple of months of figuring things out, by the fall semester I was well into
my research.Chase Van Baalen, my major professor-a gem! |
My PhD research started with purified
spinach Rubisco followed by isolating and purifying this enzyme from various
microorganisms. The idea behind this work was to figure out why carbon isotope
measurements of modern and ancient sediments and rocks were so different from
one another. Precambrian stromatolites were thought to be microbially produced
structures formed primarily by cyanobacterial primary producers. One question
that persisted was whether or not the Rubisco from cyanobacteria produced the
same degree of carbon isotope fractionation as the enzyme from higher plants.
At the time, Tabita and others thought that the cyanobacterial enzyme had a
different structural form from the higher plant Rubsico, although subsequently
this was shown not to be the case. The photosynthetic bacteria, Rhodospirillum
rubrum, produced two different forms of the enzyme: form I, the high molecular
weight form, and form II, a Rubisco with a smaller molecular weight. We
wondered whether the different forms might be associated with different
enzymatic mechanisms and consequently different isotope fractionations.
At the time Rubisco isotope research was a
very hot field. A group from New Zealand published experiments using soybean
enzyme, which showed little effect of bicarbonate concentrations or temperature
on fractionation. A team led by Bill Sackett at Texas A&M published work
using sorghum Rubisco and measured extremely variable carbon isotope
fractionations. We did not find this work compelling given the large
differences between the two temperature treatments. Further, Sackett’s group
used a cell-free extract, but not purified protein. A French group conducted
experiments that produced results having a wide range in values that was
completely beyond that known measurements in natural samples. Neither of these
groups used Rubisco from taxonomic groups other than higher plants.
My challenge was to grow microorganisms
in large quantities from liters of pure cultures and to purify the Rubisco to
homogeneity, i.e., isolating it from all other proteins. The work required that
I learn sterile technique and microbial culturing. Once the organisms had been
harvested, enzyme purification required column chromatography using size
exclusion principles. The location of Rubisco separated by chromatography was
assayed by radioactive bicarbonate (14C) uptake measurements. Once
the Rubisco was identified, I purified large amounts, milligrams of pure
protein, for the isotope experiments. Rubisco was incubated with milligram
quantities of the substrate RuBP with an excess of bicarbonate in a buffered
solution. When all of the RuBP was used up, each of the 40 experiments was
terminated and the product, phosphoglyceric acid (PGA) was purified by
crystallization.
Once the PGA was purified, I proceeded
to Pat Parker’s aging “Craig line”—a variation of hand-blown glass tubing
modified from Harmon Craig’s original vacuum system. My sample was then pushed
into a furnace in which oxygen was added, the PGA was combusted, and CO2 was
produced. The carbon isotope combustion
of carbon dioxide was analyzed on a 1960s version Nuclide 6” isotope ratio mass
spectrometer (IRMS) complete with now archaic electronic devices and strip
chart recorders for recording the isotope compositions. In those days,
measurements were completed by hand using rulers and slide rules to calculate
the isotope values. Our work showed that different enzyme forms and different
metal cofactors resulted in variations in isotope patterns. We also showed that the enzymatic isotope
change between the first product of photosynthesis and carbon dioxide was
greater than that measured in vivo with whole plants and cells. My
research still provides the foundations of understanding the evolution of
photosynthesis on earth, how climate cycles have changed over time, and how
plants manage their water budgets.
My life in Texas continued on two
planes—the academic, quiet university life and a rambling, often disorganized
personal side. I learned years later that I was known as the “Phantom” at the
Marine Science Institute. In the community, I was treated as a responsible
nerd. I kept the books and did the ordering for the ice cream business, worked
the hotel desk Friday through Sunday, and continued with my eyeglass lens
jewelry business selling in local tourist shops. Dedicated work in the lab made
the next two years fly by. I was finishing up my dissertation by April 1977, less
than three years after beginning grad school. These days a three-year PhD is
nearly unheard of. However, I had accomplished more than I had proposed. I
defended my work in Austin in mid-May.
Early on the morning of my
defense, professors Parker and Van Baalen came by with fellow student Brian
Fry, who was defending his Masters degree that same day, and together in the
Institute’s station wagon we drove 150 miles north to Austin. I presented my
dissertation work to my committee that included Parker, Van Baalen, Bob Tabita,
Jerry Brand (a botanist), and Knut Schmidt Nielson (a famous animal
physiologist). It was a fairly cut and
dry exam, over in two hours. We drove straight back to Port Aransas in late
afternoon. When I arrived, no one greeted me to hear the good news. Typically,
newly minted PhDs are regaled with a party with their friends organized by
spouses. The lack of any celebration was one of the first major warning signals
that Jack harbored jealousy over the success of my new career. In 6 weeks, we
packed up an old trailer, hitched it to the back of a rusted out station wagon
and drove north to Washington DC to start the postdoc at the Carnegie’s
Geophysical Laboratory.